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BluePippin Operations Manual 460013 Rev E 1-2 1.2 How the System Works The BluePippin systems use pre-cast and disposable agarose gel cassettes. The Paenibacillus sp. strain B-LR circular genome is 6,694,765 bp long with a 46.76% GC content and contains 6,129 coding sequences (CDSs), 81 tRNAs, and 30 rRNAs. The sequence was submitted to antiSMASH analysis of secondary metabolite biosynthesis gene clusters ( 8 ). Among the 42 clusters identified, 18 were only putative, whereas 25 were ...The Blue Pippin facilitates library construction for most popular NGS platforms, and is recommended by Pacific Biosciences, Illumina and Ion Torrent for certain workflows. Utilizing pulsed-field electrophoresis it is an ideal solution for resolving and collecting high molecular weight DNA up to 50kb. The platform features the ability to collect ...

Building on the success of the Pippin Prep and Blue Pippin size selection technologies, the PippinHT provides the user with the ability to size select up to 24 samples. Sample Flexibility Like the Blue Pippin, the PippinHT has pulsed field capabilities, thus enabling the size selection of DNA fragments intended for use in both short-read ...Buy Sea Women's Blue Pippin Smocked Patchwork-printed Cotton Maxi Apron Dress. Similar products also available. SALE now on!Pippin Hill Farm & Vineyards 2015 Easton Blue; Pippin Hill Farm & Vineyards 2019 Cabernet Sauvignon; Pippin Hill Farm & Vineyards 2019 Chardonnay Reserve; Pippin Hill Farm & Vineyards 2019 Wild Common Reserve; Pippin Hill Farm & Vineyards 2020 Chardonnay; Pippin Hill Farm & Vineyards 2020 Reserve Rosé; Pollak Vineyards 2019 Petit Verdot ...May 2, 2019 · Good quality library: A good quality library will result in most of the pores being in the “Sequencing” state (neon green), and very few in “Pore” (green), “Recovering” (dark blue), or “Inactive” (light blue). A library that results in a Duty Time graph like the example below is likely to give a good sequencing throughput. Run times are as little as 30 minutes. View the Pippin Prep cassette reference chart to explore the DNA size selection options and run times. 3% agarose, 100 - 250 bp. 2% agarose, 100 - 600 bp. 1.5% agarose, 250 bp - 1.5 kb.

The core facility staff will wear face mask in the laboratory, which hosts multiple instruments for shared use by researchers via scheduling. Users are required to follow the University's COVID-19 Policies and Guidance on this web page: The primary contact for the Genomics Core Facility is Wei Wang, the Director, [email protected], 609-258-6998.PippinHT Quick Guide HTC2010 Marker 20B. 1MB. PippinHT Quick Guide HTC1510 Marker 15C. 1MB. PippinHT Quick Guide HPE7510 Marker 75E 6-10Kb and 15-20kb High Pass. 948KB. PippinHT Quick Guide HPF7510 Marker 75F >30-40kb High Pass. 1MB. PippinHT Quick Guide HRT7510 9-30kb Range +T Marker 75E.The end-repaired fragments were size-selected using the Blue Pippin Size-Selection System (Sage Science, MA, USA), and damage-repaired using the SMRTbell Damage Repair Kit (PacBio). ….

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The pooled libraries were then cleaned up with 0.45X AMPure PB beads (Pacific Biosciences) and subjected to size-selection on Blue Pippin (SAGE Science) in order to remove DNA fragments < 7 kb. The completed 20-22-plex pools were annealed to sequencing primer V4 and bound to sequencing polymerase 2.0, and were sequenced using one SMRTcell 8 M ...Gel free size selection of libraries was performed using Blue pippin. Traces of amplified and size selected libraries were assessed using Bioanalyzer 2100 (Agilent technologies). The libraries were denatured and diluted according to the manufacturer’s protocols using NextSeq 500/550 High output kit v2 (75 cycles). Sequencing was carried out ...selected using the Blue Pippin (Sage Science, United Kingdom). Illumina libraries were constructed with a PCR-free method. using NEBNext End Repair, NEBNext dA-t ailing and Blunt T/A.

selected using the Blue Pippin (Sage Science, United Kingdom). Illumina libraries were constructed with a PCR-free method. using NEBNext End Repair, NEBNext dA-t ailing and Blunt T/A.First-strand cDNA was synthesized using a SMARTer PCR cDNA Synthesis Kit (Clontech, CA, United States). After a few rounds of PCR amplification, the amplified cDNA was divided into different size fractions to prevent preferential small template sequencing using a Blue Pippin (Sage Science, MA, United States).Background Orius laevigatus, a minute pirate bug, is a highly effective beneficial predator of crop pests including aphids, spider mites and thrips in integrated pest management (IPM) programmes. No genomic information is currently available for O. laevigatus, as is the case for the majority of beneficial predators which feed on crop …

gospel legend with the hit crossword clue To generate Nanopore long reads, ~15 µg of genomic DNA was size-selected (>20 Kb) with a Blue Pippin System (Sage Science). According to 1D Genomic DNA by Ligation protocol (Oxford Nanopore Technologies, ONT), ONT SQK-LSK108 library preparation kit was used to construct PCR-free libraries. Nextomics (Nextomic Biosciences Technologies ...After this, PCR reamplification of the captured DNA pool with spacer-acquisition-specific primers allows visible detection of spacer acquisition in just 1 in 10 5 cells (0.001%) in the population ... jim derryku sonography program BluePippin and Pippin HT. The Pippin systems from SageScience allow for precise automated size selection of nucleic acid samples, sequencing libraries, and even protein samples. Our Core runs both the BluePippin and the Pippin HT.Services - BluePippin (SageScience) • BluePippin Cassette kits • Online Submission Request • Physical Submission of Samples. The BluePippin (jpg) has the capabilities of the Pippin Prep, plus pulsed-field electrophoresis for resolving and collecting high molecular weight DNA. Target sizes or ranges of sizes are entered in software, and fractions are collected in buffer with no ... ase 2022 Auntie Mabel & Pippin Homage Sweatshirt. 1 review. Regular price £33.00 Sale price £26.99 Save 18 ... Blue"],"price":2699,"weight":399,"compare_at_price":3300 ...Services - BluePippin (SageScience) • BluePippin Cassette kits • Online Submission Request • Physical Submission of Samples. The BluePippin (jpg) has the capabilities of the Pippin Prep, plus pulsed-field electrophoresis for resolving and collecting high molecular weight DNA. Target sizes or ranges of sizes are entered in software, and fractions are collected in buffer with no ... wvu vs kansas footballkansas 2021 basketball scheduleartist presentation Blue Pippin Size Selection. Blue Pippin Sample Submission Form. Blue Pippin Submission Template; Library Preparation. We offer standard library preparation for RNA-Seq, microRNA, and whole genome sequencing samples. For other sequencing types, we can assist you with designing your protocol and can work with you as needed to sequence your samples. how did african american contribute to ww2 Find the nearest stores. Search Address. Use current location. Seiko Prospex remains a standard for high-intensity timekeeping excellence. From the introduction of its first diver’s watch in 1965, Seiko has been a leader in undersea precision and durability, spearheading innovations in both technology and design.Finally, large fragments (>10 kb) were enriched with Blue Pippin device (Sage Science, Inc., Beverly, MA, USA) for sequencing. A total of 15 single-molecule real-time (SMRT) cells were processed, 7 with Sequel and 8 with RS II instruments (PacBio), to generate a total of 67.32 Gb PacBio data. For Oxford Nanopore sequencing, ∼5 μg of genomic ... platoclosetmale massage rentwhat was the first insect on earth DNA fragments were amplified by PCR twice and size selected (300-450 bp) using a Blue Pippin. The final library was sequenced using a single-end 100 bp protocol within a lane of an Illumina Hiseq-4000 sequencer at the University of Texas at Austin Genome Sequencing and Analysis Facility (GSAF).The ~500 bp insert size libraries were sequenced on Illumina HiSeq 2500 (2 × 125 PE). The 3 DNA candidates were also sent for PacBio library construction. To do this, 10 μg gDNA was sheared to an ~20-kb targeted size using a Covaris g-Tube, then used for size selection using Blue Pippin (Sage Science, Beverly, MA, USA).